maize chlorotic mottle virus

 Summary

Maize chlorotic mottle virus causes lethal necrosis of corn and can be transmitted through infected corn seed. It is still challenging to detect this virus to prevent its introduction, infection and transmission in all areas. For this purpose, the Maize chlorotic mottle virus was visually investigated using unmodified gold nanoparticles (AuNPs). The detection is based on the fact that the dispersed AuNP solution is red due to the strong plasmon absorption band at 520 nm, while the AuNP solution in the presence of species-specific and RT-PCR products of Maize chlorotic mottle virus is grey. Blue after saline infusion. After optimizing the size of AuNPs, the concentration of NaCl, the investigation of the reaction system and the analysis of the specificity and sensitivity of the new test, the detection of Maize chlorotic mottle virus by the use of unmodified AuNPs was carried out by Easy preparation of samples from us. Study. With this assay, less than 30 pg μL−1 of Maize chlorotic mottle virus RNA was detected visually, with the naked eye, without the need for any complex, expensive equipment and reagents of biochemical. The specificity was 100%, and the assay showed good reproducibility on 15 sample viruses. The results show that this assay is species-specific, simple, inexpensive, and readily detectable for the detection of Maize chlorotic mottle virus in plant tissue. Therefore, detecting the Maize chlorotic mottle virus is a useful tool for medium or small companies and screening and isolating Maize chlorotic mottle virus in seed corn or plant cells.

Pathogen

Maize Chlorotic Mottle Virus (Sobemovirus). Virus structures: a single isometric particle containing a single RNA. This virus occurs in South Central Nebraska, North Central Kansas, and South America. How the virus lives and the main source of the inoculum are unknown. Certain beetles' larvae and adult stages, including the corn rootworm, transmit the virus.

Symptoms of Disease

Hybrids and inbreds vary in sensitivity to MCMV. Early symptoms can be troublesome to distinguish from malnutrition or maize genotype-environment interactions. As the disease progresses, the symptoms of the leaves. The ears of highly susceptible hybrids do not give well; kernel size and number are affected. A mixed infection of MCMV with Maize Dwarf Mosaic Virus or Wheat Streak Mosaic Virus afford result in Corn Lethal Necrosis.

Good Environmental Conditions

High temperatures and long periods of wet weather favour leaf recovery and high levels.

High temperature and plant stress after pollination favour the stalk decay stage.

Administration

Genetic Resistance

Resistant hybrids are commercially available and are the best management option.

Cultural Practices

Tillage reduces the risk of residue incorporation into the soil and the effects of decomposition. Crop rotation to other crops for the year reduces the incidence of MCMV in the following corn crop.